TOP2B Polyclonal antibody

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  WB analysis of HeLa using 20549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20549-1-AP

  WB result of TOP2B antibody (20549-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.

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  WB analysis of HeLa using 20549-1-AP

  WB result of TOP2B antibody (20549-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HeLa cells.

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  WB analysis of HepG2 using 20549-1-AP

  WB result of TOP2B antibody (20549-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.

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  WB analysis of HepG2 using 20549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20549-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20549-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 20549-1-AP

  IP result of anti-TOP2B (IP:20549-1-AP, 4ug; Detection:20549-1-AP 1:300) with HepG2 cells lysate 3200ug.

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  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 20549-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 20549-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (20549-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 20549-1-AP

  Immunofluorescent analysis of HepG2 cells, using TOP2B antibody 20549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20549-1-AP

Publications(14)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

DNA topoisomerase 2 beta, TOP2B, top2beta, TOPIIB

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Product Information

20549-1-AP targets TOP2B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: topoisomerase (DNA) II beta 180kDa
• Calculated Molecular Weight: 183 kDa
• Observed Molecular Weight: 183 kDa
• GenBank Accession Number: NM_001068
• Gene Symbol: TOP2B
• Gene ID (NCBI): 7155
• RRID: AB_10700004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02880
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Science; Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.; Authors - Anand Minajigi
human	WB,IP	Mol Cell; MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".; Authors - Subhendu K Das
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou
human	WB	RNA; DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells; Authors - Maya David
mouse	WB	Sci Rep; Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.; Authors - Fei Xu ; KD Validated
human		Sci Rep; Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.; Authors - Lin-Yang Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (02-24-2025)

Antibody works really well and recognizes a band corresponding to TOP2B.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293