TOR1AIP1 Polyclonal antibody

TOR1AIP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21459-1-AP

Synonyms

LAP1B, LAP1, Torsin-1A-interacting protein 1, TOR1AIP 1, LGMD2Y

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Validation Data Gallery

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  WB analysis using 21459-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21459-1-AP (TOR1AIP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 21459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOR1AIP1 antibody (21459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, HEK-293T cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

21459-1-AP targets TOR1AIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOR1AIP1 fusion protein Ag14823
• Full Name: torsin A interacting protein 1
• Calculated Molecular Weight: 462aa,52 kDa; 583aa,66 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC023247
• Gene Symbol: TOR1AIP1
• Gene ID (NCBI): 26092
• RRID: AB_2878861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Elife; CDK1 controls CHMP7-dependent nuclear envelope reformation.; Authors - Alberto T Gatta
mouse,human	WB,IF,IP	Biochem Biophys Res Commun; Analysis of the GFP-labelled β-dystroglycan interactome in HEK-293 transfected cells reveals novel intracellular networks; Authors - Francesca Sciandra
human	WB,IF	Nat Cell Biol; LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion; Authors - Yaiza Jung-Garcia