TORC2 Recombinant antibody

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  WB analysis using 85150-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85150-4-RR (CRTC2,TORC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 85150-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TORC2 Recombinant antibody (85150-4-RR, Clone:242808B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 85150-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85150-4-RR against Human TORC2 were performed. The affinity constant is 0.422 nM.

TORC2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 85150-4-RR

Clone No.242808B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CRTC2, CRTC2,TORC2, 242808B6, CREB-regulated transcription coactivator 2, TORC 2

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, Raji cells, A-673 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85150-4-RR targets TORC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TORC2 fusion protein Ag3167
• Full Name: CREB regulated transcription coactivator 2
• Calculated Molecular Weight: 693 aa, 73 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC053562
• Gene Symbol: TORC2
• Gene ID (NCBI): 200186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q53ET0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .