TOX Polyclonal antibody

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• TOX Polyclonal antibody (34301-1-AP)

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  WB analysis using 34301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 34301-1-AP (TOX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

TOX Polyclonal Antibody for WB, ELISA

Cat No. 34301-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KIAA0808, Thymocyte selection-associated high mobility group box protein TOX, TOX1

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

34301-1-AP targets TOX in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag41257

  Product name: Recombinant human TOX protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 50-235 aa of BC016665

  Sequence: DYVPASQSYPGPSLESEDFNIPPITPPSLPDHSLVHLNEVESGYHSLCHPMNHNGLLPFHPQNMDLPEITVSNMLGQDGTLLSNSISVMPDIRNPEGTQYSSHPQMAAMRPRGQPADIRQQPGMMPHGQLTTINQSQLSAQLGLNMGGSNVPHNSPSPPGSKSATPSPSSSVHEDEGDDTSKINGG

• Full Name: thymocyte selection-associated high mobility group box
• Calculated Molecular Weight: 526 aa, 58 kDa
• Observed Molecular Weight: 57~70 kDa
• GenBank Accession Number: BC016665
• Gene Symbol: TOX
• Gene ID (NCBI): 9760
• RRID: AB_3743187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O94900
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thymocyte selection-associated high-mobility group box (TOX) is a DNA-binding factor that can regulate transcription by modifying local chromatin structure and modulating the formation of multi-protein complexes. TOX has multiple roles in the development of the adaptive immune system including development of CD4 T cells, NK cells and lymph node organogenesis (PMID: 35485425). Two bands detected at ~63 kDa and ~57kDa were presumed to be the unmodified form and the post-translationally form of TOX, respectively,(PMID: 32106280).