TOX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TOX2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21162-1-AP

Synonyms

C20orf100, GCX 1, GCX1, TOX2

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Validation Data Gallery

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  WB analysis using 21162-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21162-1-AP (TOX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 21162-1-AP

  IP result of anti-TOX2 (IP:21162-1-AP, 4ug; Detection:21162-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human breast cancer using 21162-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21162-1-AP (TOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21162-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21162-1-AP (TOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 21162-1-AP is TOX2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOX2 fusion protein Ag14772
• Full Name: TOX high mobility group box family member 2
• Calculated Molecular Weight: 488 aa, 52 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007636
• Gene Symbol: TOX2
• Gene ID (NCBI): 84969
• RRID: AB_2878821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells.; Authors - Shu Horiuchi ; KO Validated
human	WB,IF,ChIP	Mol Cancer; Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma; Authors - Jianbiao Zhou ; KD Validated