• Featured Product
  • KD/KO Validated

TOX2 Polyclonal antibody

TOX2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 21162-1-AP

Synonyms

C20orf100, GCX 1, GCX1, TOX2



Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21162-1-AP is TOX2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOX2 fusion protein Ag14772
Full Name TOX high mobility group box family member 2
Calculated Molecular Weight 488 aa, 52 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007636
Gene Symbol TOX2
Gene ID (NCBI) 84969
RRIDAB_2878821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

Protocols

Product Specific Protocols
WB protocol for TOX2 antibody 21162-1-APDownload protocol
IHC protocol for TOX2 antibody 21162-1-APDownload protocol
IP protocol for TOX2 antibody 21162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells.

Authors - Shu Horiuchi
  • KO Validated
humanWB,IF,ChIP

Mol Cancer

Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma

Authors - Jianbiao Zhou
  • KD Validated