TP53BP1-Specific Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83809-1-RR (same clone as 83809-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83809-1-RR (same clone as 83809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (83809-1-RR, Clone: 240638C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TP53BP1-Specific Recombinant antibody (83809-1-RR, Clone:240638C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

TP53BP1-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 83809-1-PBS

Clone No.240638C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424

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Product Information

83809-1-PBS targets TP53BP1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor protein p53 binding protein 1
• Calculated Molecular Weight: 214 kDa
• Observed Molecular Weight: 450 kDa
• GenBank Accession Number: NM_005657
• Gene Symbol: TP53BP1
• Gene ID (NCBI): 7158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q12888
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage.