Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16596-1-AP targets TP53I13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9746 Product name: Recombinant human TP53I13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC001593 Sequence: MAPPPPSPQLLLLAALARLLGPSEVMAGPAEEAGAHCPESLWPLPPQVSPRVTYTRVSPGQAEDVTFLYHPCAHPWLKLQLALLAYACMANPSLTPDFSLTQDRPLVLTAWGLALEMAWVEPAWAAHWLMRRRRRKQRKKKAWIYCESLSGPAPSEPTPGRGRLCRRGCVQALALAFALRSWRPPGTEVTSQGPRQPSSSGAKRRRLRAALGPQPTRSALRFPSASPGSLKAKQSMAGIPGRESNAPSVPTVSLLPGAPGGNASSRTEAQVPNGQGSPGGCVCSSQASPAPRAAAPPRAARGPTPRTEEA Predict reactive species |
| Full Name | tumor protein p53 inducible protein 13 |
| Calculated Molecular Weight | 393 aa, 42 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC001593 |
| Gene Symbol | TP53I13 |
| Gene ID (NCBI) | 90313 |
| RRID | AB_3085499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBR0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TP53I13 is involved in several processes, including negative regulation of cell cycle, response to UV, and response to xenobiotic stimulus.It may act as a tumor suppressorwhich could inhibits tumor cell growth, when overexpressed.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TP53I13 antibody 16596-1-AP | Download protocol |
| WB protocol for TP53I13 antibody 16596-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |





