TP53INP1 Polyclonal antibody

TP53INP1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17872-1-AP

Synonyms

p53DINP1, SIP, Stress induced protein, Teap, TP53DINP1, TP53INP1, TP53INP1A, TP53INP1B

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Validation Data Gallery

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  IHC staining of human liver cancer using 17872-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17872-1-AP (TP53INP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17872-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17872-1-AP (TP53INP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17872-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TP53INP1 antibody (17872-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17872-1-AP targets TP53INP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TP53INP1 fusion protein Ag12358
• Full Name: tumor protein p53 inducible nuclear protein 1
• Calculated Molecular Weight: 240 aa, 27 kDa
• GenBank Accession Number: BC074868
• Gene Symbol: TP53INP1
• Gene ID (NCBI): 94241
• RRID: AB_2878458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Biochem Biophys Res Commun; Exosomal miR-93 promotes proliferation and invasion in hepatocellular carcinoma by directly inhibiting TIMP2/TP53INP1/CDKN1A.; Authors - Xiaofeng Xue
human		Int J Mol Sci; TP53inp1 Gene Is Implicated in Early Radiation Response in Human Fibroblast Cells.; Authors - Nikolett Sándor
human		Exp Ther Med; PMID: 31410122; Authors - Aijun Ren