• Featured Product
  • KD/KO Validated

p63 Polyclonal antibody

p63 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12143-1-AP

Synonyms

AIS, B(p51A), B(p51B), CUSP, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like



Tested Applications

Positive WB detected inA431 cells, human heart tissue, mouse heart tissue, U-937 cells, Apoptosised HeLa cells, HaCaT cells, PC-12 cells, mouse thymus tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman tonsillitis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, human lung cancer tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12143-1-AP targets p63 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p63 fusion protein Ag2791
Full Name tumor protein p63
Calculated molecular weight 680 aa, 77 kDa
Observed molecular weight 63-68 kDa
GenBank accession numberBC039815
Gene symbol TP63
Gene ID (NCBI) 8626
RRIDAB_10597397
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Protocols

Product Specific Protocols
WB protocol for p63 antibody 12143-1-APDownload protocol
IHC protocol for p63 antibody 12143-1-APDownload protocol
IF protocol for p63 antibody 12143-1-APDownload protocol
IP protocol for p63 antibody 12143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanWB,IP,ChIP

Nucleic Acids Res

Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.

Authors - Yongsheng Huang
  • KD Validated
humanIF

Cell Rep

A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma

Authors - Yue Li
mouse

Oncogene

TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice.

Authors - Samera H Hamad
humanIF

Elife

Chloride channels regulate differentiation and barrier functions of the mammalian airway.

Authors - Mu He
human

Oncogene

Circulating IGF-1 promotes prostate adenocarcinoma via FOXO3A/BIM signaling in a double-transgenic mouse model.

Authors - Shuang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Kui (Verified Customer) (09-19-2019)

Best P63 Ab I can find so far.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Esophagus
p63 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse Esophagus (Cat no:12143-1-AP)