TPBG Polyclonal antibody

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• TPBG Polyclonal antibody (29394-1-AP)

TPBG Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29394-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein

• 

  WB analysis using 29394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29394-1-AP (TPBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse eye using 29394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse eye using 29394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30019

  Product name: Recombinant human TPBG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 153-355 aa of NM_006670

  Sequence: PLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS

• Full Name: trophoblast glycoprotein
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: NM_006670
• Gene Symbol: TPBG
• Gene ID (NCBI): 7162
• RRID: AB_2918290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13641
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)

Publications

What published studies show

The TPBG polyclonal antibody (Cat# 29394-1-AP) has 2 citations. These appear in Journal of Cellular and Molecular Medicine (2025), examining TPBG as a predictive indicator for gastric cancer using WB, and Cancer Management and Research (2022), investigating MIR31HG in colorectal cancer progression using IHC. Both studies focus on human samples and relate to oncology research, specifically gastric and colorectal cancer biomarker discovery and prognostic evaluation.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Cell Mol Med; In-Depth Examination of TPBG as a New Predictive Indicator for Gastric Cancer; Authors - Lianlei Yang
human	IHC	Cancer Manag Res; MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression.; Authors - Jianlong Wang