TPBG Polyclonal antibody

TPBG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29394-1-AP

Synonyms

5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein

---

Validation Data Gallery

• 

  WB analysis using 29394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29394-1-AP (TPBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 29394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 29394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPBG fusion protein Ag30019
• Full Name: trophoblast glycoprotein
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: NM_006670
• Gene Symbol: TPBG
• Gene ID (NCBI): 7162
• RRID: AB_2918290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)

Publications

Species	Application	Title
human	IHC	Cancer Manag Res; MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression.; Authors - Jianlong Wang