TPH2 Polyclonal antibody

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  WB analysis using 29283-1-AP

  Rat brain tissue were subjected to SDS PAGE followed by western blot with 29283-1-AP (TPH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29283-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 29283-1-AP (TPH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TPH2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29283-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:1.14.16.4, NTPH, TPH 2, Tryptophan 5-hydroxylase 2, Tryptophan 5-monooxygenase 2

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29283-1-AP targets TPH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPH2 fusion protein Ag30281
• Full Name: tryptophan hydroxylase 2
• Calculated Molecular Weight: 490 aa, 56 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC114442
• Gene Symbol: TPH2
• Gene ID (NCBI): 121278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWU9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPH2 protein is a key enzyme in the synthesis of serotonin in the brain. It is mainly expressed in the brain and is responsible for converting tryptophan to 5-hydroxytryptophan, a precursor of serotonin. TPH2 plays a crucial role in regulating mood, anxiety, and stress responses. Variations in the TPH2 gene have been associated with several psychiatric disorders, including depression and bipolar disorder. Additionally, TPH2 expression can be regulated by various factors, such as hormones and environmental stimuli (PMID: 22241550, PMID: 29775696, PMID: 32119710, PMID: 40204433).