TPI1 Monoclonal antibody

• 

  WB analysis of HEK-293 using 60036-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60036-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60036-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

TPI1 Monoclonal Antibody for WB, ELISA

Cat No. 60036-2-Ig

Clone No.3G11G3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60036-2-Ig targets TPI1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1175

  Product name: Recombinant human TPI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-249 aa of BC007086

  Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

• Full Name: triosephosphate isomerase 1
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC007086
• Gene Symbol: TPI1
• Gene ID (NCBI): 7167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P60174
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).