TPK1 Polyclonal antibody

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TPK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10942-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, PP20, Placental protein 20, hTPK1

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  WB analysis using 10942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 10942-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10942-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 10942-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse small intestine using 10942-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 10942-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human small intestine using 10942-1-AP

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 10942-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 10942-1-AP

  IP result of anti-TPK1 (IP:10942-1-AP, 4ug; Detection:10942-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10942-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, human small intestine tissue, mouse testis tissue, L02 cells, mouse small intestine tissue, mouse kidney tissue, rat small intestine tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10942-1-AP targets TPK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1404

  Product name: Recombinant human TPK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 11-243 aa of BC014552

  Sequence: LLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS

• Full Name: thiamin pyrophosphokinase 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC014552
• Gene Symbol: TPK1
• Gene ID (NCBI): 27010
• RRID: AB_2205647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H3S4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).

Publications

What published studies show

TPK1 Polyclonal antibody (Cat# 10942-1-AP) has 12 citations across journals including Redox Biology, Science Advances, PNAS, Oncotarget, Pharmaceuticals, Biological Research, Scientific Reports, American Journal of Pathology, American Journal of Physiology–Renal Physiology, Immunology Letters, and PLoS ONE. Research fields span thiamine metabolism, oxidative stress, cancer (leukemia, melanoma, breast cancer), neurobiology, renal physiology, rheumatoid arthritis, and pancreatic cell biology.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Redox Biol; Clioquinol inactivates thiamine pyrophosphate by increasing cellular oxidative stress.; Authors - Zheyu Fan
human	WB	Sci Adv; Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells.; Authors - Rohiverth Guarecuco ; KO Validated
mouse	WB	Proc Natl Acad Sci U S A; OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.; Authors - Ligong Chen
human	WB	Oncotarget; Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen.; Authors - Gaganpreet S Tiwana
human	WB	Pharmaceuticals (Basel); Chlorogenic Acid Induced Neuroblastoma Cells Differentiation via the ACAT1-TPK1-PDH Pathway; Authors - Shen You
rat	WB	Biol Res; Thiamine deficiency contributes to synapse and neural circuit defects.; Authors - Qiujian Yu