TPM3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TPM3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10737-1-AP

Synonyms

Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin

---

Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10737-1-AP

  WB result of TPM3 antibody (10737-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM3 transfected HepG2 cells.

• 

  WB analysis using 10737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10737-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10737-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10737-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10737-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 10737-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10737-1-AP

  IP result of anti-TPM3 (IP:10737-1-AP, 4ug; Detection:10737-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human colon cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10737-1-AP (TPM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue, human heart tissue, HeLa cells, HEK-293 cells, rat heart tissue, HepG2 cells, rat skeletal muscle tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10737-1-AP targets TPM3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPM3 fusion protein Ag1171
• Full Name: tropomyosin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC008407
• Gene Symbol: TPM3
• Gene ID (NCBI): 7170
• RRID: AB_2205671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tropomyosins are ubiquitous proteins of 35 to 45 kDa associated with the actin filaments of myofibrils and stress fibers. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins.TPM2 subunit constitutes one part of the TPM dimer, and TPM1 or TPM3 constitutes the other part of the TPM dimer (22064019), causing an extra band of ~70kDa.

Publications

Species	Application	Title
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
	IP	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada
human	WB	Oncogene; MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.; Authors - Agarwal N N
human,mouse	WB	Oncol Res; LncRNA WEE2-AS1 Knockdown Inhibits the Proliferation, Migration and 3 Invasion of Glioma Cells via Regulating miR-29b-2-5p/TPM3 Axis.; Authors - Zhen Jia
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin
human	IHC	Bioengineered; PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma.; Authors - Kaiming Peng