TPM3 Monoclonal antibody

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  WB analysis using 68158-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 68158-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of C2C12 using 68158-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TPM3 antibody (68158-1-Ig, Clone: 1F10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

TPM3 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68158-1-Ig

Clone No.1F10B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin

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Tested Applications

• Positive WB detected in: pig heart tissue, U2OS cells, Rabbit heart tissue, Rat heart tissue, Mouse heart tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68158-1-Ig targets TPM3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28568

  Product name: Recombinant human TPM3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-285 aa of BC008407

  Sequence: MMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISEELDHALNDMTSI

• Full Name: tropomyosin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33-37 kDa
• GenBank Accession Number: BC008407
• Gene Symbol: TPM3
• Gene ID (NCBI): 7170
• RRID: AB_3085044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P06753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.