TPM4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TPM4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E12D12

Cat no : 67244-1-PBS

Synonyms

TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain

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Validation Data Gallery

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  WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

  WB result of TPM4 antibody (67244-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected A549 cells. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67244-1-PBS targets TPM4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TPM4 fusion protein Ag6947
• Full Name: tropomyosin 4
• Calculated Molecular Weight: 248 aa, 29 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC037576
• Gene Symbol: TPM4
• Gene ID (NCBI): 7171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.