TPPP Polyclonal antibody

• 

  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of Neuro-2a using 18742-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TPPP antibody (18742-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

TPPP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18742-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

25 kDa brain specific protein, 25 kDa brain-specific protein, EC:3.6.5.-, p24, p25 alpha

---

Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

18742-1-AP targets TPPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tubulin polymerization promoting protein
• Calculated Molecular Weight: 219 aa, 24 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC131506
• Gene Symbol: TPPP
• Gene ID (NCBI): 11076
• RRID: AB_2256275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94811
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cell; The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for Myelination.; Authors - Meng-Meng Fu
human	WB,IHC,IF	FASEB J; EBV-miR-BART12 accelerates migration and invasion in EBV-associated cancer cells by targeting tubulin polymerization-promoting protein 1.; Authors - Yingfen Wu
human	WB	Stem Cells Int; Enhanced Effect of IL-1β-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling.; Authors - Liu Liu
	WB	ACS Infect Dis; Repositioning Brusatol as a Transmission Blocker of Malaria Parasites; Authors - Amelia Cox
rat	WB	J Mol Cell Cardiol; Defective autophagy triggered by arterial cyclic stretch promotes neointimal hyperplasia in vein grafts via the p62/nrf2/slc7a11 signaling pathway; Authors - Yi Chen