TPR Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83177-5-RR (same clone as 83177-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83177-5-RR (TPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83177-5-RR (same clone as 83177-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83177-5-RR (TPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83177-5-RR (same clone as 83177-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TPR antibody (83177-5-RR, Clone: 230413A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

TPR Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83177-5-PBS

Clone No.230413A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nucleoprotein TPR, 230413A2

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Product Information

83177-5-PBS targets TPR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: translocated promoter region (to activated MET oncogene)
• Calculated Molecular Weight: 267 kDa
• Observed Molecular Weight: ~270 kDa
• GenBank Accession Number: NM_003292
• Gene Symbol: TPR
• Gene ID (NCBI): 7175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P12270
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065).