Tested Applications
| Positive WB detected in | human heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12800-1-AP targets TPRKB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3551 Product name: Recombinant human TPRKB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC029492 Sequence: MQLTHQLDLFPECRVTLLLFKDVKNAGDLRRKAMEGTIDGSLINPTVIVDPFQILVAANKAVHLYKLGKMKTRTLSTEIIFNLSPNNNISEALKKFGISANDTSILIVYIEEGEKQINQEYLISQVEGHQVSLKNLPEIMNITEVKKIYKLSSQEESIGTLLDAIICRMSTKDVL Predict reactive species |
| Full Name | TP53RK binding protein |
| Calculated Molecular Weight | 175 aa, 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC029492 |
| Gene Symbol | TPRKB |
| Gene ID (NCBI) | 51002 |
| RRID | AB_2256289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3C4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TPRKB, also named as CGI 121, belongs to the CGI121/TPRKB family. It interacts with TP53RK/PRPK and is widely expressed in all tissues. This antibody is a rabbit polyclonal antibody raised against full length TPRKB of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPRKB antibody 12800-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TPRKB Polyclonal antibody (Cat# 12800-1-AP) has 1 total citation. It was cited in Experimental Cell Research (2024, IF 3.5) in a study investigating how METTL5 enhances TPRKB mRNA stability via m6A modification to promote aggressive phenotypes in hepatocellular carcinoma cells. The research falls within the fields of RNA epigenetics and liver cancer biology. The antibody was used for WB and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Exp Cell Res METTL5 enhances the mRNA stability of TPRKB through m6A modification to facilitate the aggressive phenotypes of hepatocellular carcinoma cell |
