TPSAB1/TPSB2 Polyclonal antibody

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• TPSAB1/TPSB2 Polyclonal antibody (13343-1-AP)

TPSAB1/TPSB2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13343-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

TPSAB1, EC:3.4.21.59, MCP7, TPS1, TPS2

• 

  WB analysis using 13343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 13343-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 13343-1-AP

  IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, K-562 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13343-1-AP targets TPSAB1/TPSB2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4167

  Product name: Recombinant human TPSAB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-275 aa of BC028059

  Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP

• Full Name: tryptase alpha/beta 1
• Calculated Molecular Weight: 275 aa, 31 kDa
• Observed Molecular Weight: 32-38 kDa
• GenBank Accession Number: BC028059
• Gene Symbol: TPSAB1
• Gene ID (NCBI): 7177
• RRID: AB_2206197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15661
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Clin Transl Med; Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour; Authors - Kaiyuan Ji
rat	IHC	Front Immunol; Palmatine treats urticaria by reducing inflammation and increasing autophagy; Authors - Tian Xiao
		Int Immunopharmacol; Cold-inducible RNA-binding protein promotes gut barrier dysfunction and visceral hypersensitivity via activating mast cells in IBS-D; Authors - Tao Wang
human	IHC	IUBMB Life; Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma.; Authors - Weijuan Lou
human	WB	BMC Cancer; The release of tryptase from mast cells promote tumor cell metastasis via exosomes.; Authors - Hui Xiao
		Shock; Activation of intestinal mast cells contributes to gut damage after cardiac arrest in mice; Authors - Lihong Dang