Tested Applications
| Positive WB detected in | A431 cells, K-562 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13343-1-AP targets TPSAB1/TPSB2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4167 Product name: Recombinant human TPSAB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC028059 Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP Predict reactive species |
| Full Name | tryptase alpha/beta 1 |
| Calculated Molecular Weight | 275 aa, 31 kDa |
| Observed Molecular Weight | 32-38 kDa |
| GenBank Accession Number | BC028059 |
| Gene Symbol | TPSAB1 |
| Gene ID (NCBI) | 7177 |
| RRID | AB_2206197 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15661 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TPSAB1/TPSB2 antibody 13343-1-AP | Download protocol |
| WB protocol for TPSAB1/TPSB2 antibody 13343-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TPSAB1/TPSB2 Polyclonal antibody (Cat# 13343-1-AP) has 17 citations across journals including Nature Communications, Frontiers in Immunology, International Immunopharmacology, BMC Cancer, Oncology Reports, and Journal of Inflammation Research. Research fields span mast cell biology, allergic asthma, urticaria, cancer metastasis, diabetic neuropathy, gut barrier dysfunction, septic cardiomyopathy, rheumatoid arthritis, colitis, and obesity.
| Species | Application | Title |
|---|---|---|
Nat Commun Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice | ||
Clin Transl Med Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour | ||
Chin J Nat Med A new phenolic acid isolated from Salvia miltiorrhiza ameliorates OVA-induced allergic asthma by regulation of Th17/Treg cells and inflammation through the TLR4 pathway. | ||
Front Immunol Palmatine treats urticaria by reducing inflammation and increasing autophagy | ||
Int Immunopharmacol Cold-inducible RNA-binding protein promotes gut barrier dysfunction and visceral hypersensitivity via activating mast cells in IBS-D | ||
Int Immunopharmacol Edaravone attenuates cerebral inflammation by inhibiting mast cells degranulation via ROS/STIM1 signaling pathway in HIE model |
