TPSAB1 Monoclonal antibody

TPSAB1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

6A10G1

Reviews (1)

Cat no : 66174-1-Ig

Synonyms

MCP7, TPS1, TPS2, TPSAB1, TPSB1, Tryptase 1, tryptase alpha/beta 1, Tryptase beta 1, Tryptase I

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Validation Data Gallery

• 

  WB analysis of human kidney using 66174-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66174-1-Ig (TPSAB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66174-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 10x lens) microwave oven heating mediated antigen retrieved with .

• 

  IHC staining of human tonsillitis using 66174-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 40x lens) microwave oven heating mediated antigen retrieved with .

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Tested Applications

• Positive WB detected in: human kidney tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66174-1-Ig targets TPSAB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TPSAB1 fusion protein Ag4167
• Full Name: tryptase alpha/beta 1
• Calculated Molecular Weight: 275 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC028059
• Gene Symbol: TPSAB1
• Gene ID (NCBI): 7177
• RRID: AB_2881569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites.

Publications

Species	Application	Title
human	IHC	Oncogene; Mast cells inhibit colorectal cancer development by inducing ER stress through secreting Cystatin C; Authors - Feifei Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsilitis tissue. HIER with citrate buffer

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

TPSAB1 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66174-1-Ig)