TPST1 Polyclonal antibody

TPST1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12404-1-AP

Synonyms

TPST 1, TPST1

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Validation Data Gallery

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  WB analysis using 12404-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12404-1-AP (TPST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12404-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12404-1-AP (TPST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12404-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12404-1-AP (TPST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12404-1-AP targets TPST1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPST1 fusion protein Ag3093
• Full Name: tyrosylprotein sulfotransferase 1
• Calculated Molecular Weight: 370 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC013188
• Gene Symbol: TPST1
• Gene ID (NCBI): 8460
• RRID: AB_2877855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
hamster	WB	Biotechnol J; Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation.; Authors - Ren Liu