|Positive WB detected in||mouse placenta tissue, A431 cells, HepG2 cells, MCF-7 cells, mouse testis tissue, PC-3 cells|
|Positive IP detected in||human placenta tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10824-1-AP targets TPT1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TPT1 fusion protein Ag1268|
|Full Name||tumor protein, translationally-controlled 1|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22-25 kDa|
|GenBank accession number||BC003352|
|Gene ID (NCBI)||7178|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.
LncRNA TPT1-AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression.
Identification of proteins involved in neural progenitor cell targeting of gliomas.
Invest New Drugs
A novel ligand of the translationally controlled tumor protein (TCTP) identified by virtual drug screening for cancer differentiation therapy.
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AMG900 as novel inhibitor of the translationally controlled tumor protein.
Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.