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  • KD/KO Validated

TPT1 Polyclonal antibody

TPT1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10824-1-AP

Synonyms

Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1



Tested Applications

Positive WB detected inmouse placenta tissue, A431 cells, HepG2 cells, MCF-7 cells, mouse testis tissue, PC-3 cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10824-1-AP targets TPT1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPT1 fusion protein Ag1268
Full Name tumor protein, translationally-controlled 1
Calculated molecular weight 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC003352
Gene symbol TPT1
Gene ID (NCBI) 7178
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.

Protocols

Product Specific Protocols
WB protocol for TPT1 antibody 10824-1-APDownload protocol
IHC protocol for TPT1 antibody 10824-1-APDownload protocol
IP protocol for TPT1 antibody 10824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

LncRNA TPT1-AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression.

Authors - Weimin Wu
  • KD Validated
ratWB,IHC

BMC Cancer

Identification of proteins involved in neural progenitor cell targeting of gliomas.

Authors - Staflin Karin K
humanIP

Invest New Drugs

A novel ligand of the translationally controlled tumor protein (TCTP) identified by virtual drug screening for cancer differentiation therapy.

Authors - Nicolas Fischer
humanWB

Chem Biol Interact

AMG900 as novel inhibitor of the translationally controlled tumor protein.

Authors - Nicolas Fischer
humanWB

Pharmacol Res

Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.

Authors - Dongrong Zhu