Validation Data Gallery
|Positive WB detected in||A431 cells, mouse placenta tissue, MCF-7 cells|
|Positive IP detected in||human placenta tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10824-1-AP targets TPT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TPT1 fusion protein Ag1268|
|Full Name||tumor protein, translationally-controlled 1|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22-25 kDa|
|GenBank accession number||BC003352|
|Gene ID (NCBI)||7178|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.
|Product Specific Protocols|
|WB protocol for TPT1 antibody 10824-1-AP||Download protocol|
|IHC protocol for TPT1 antibody 10824-1-AP||Download protocol|
|IF protocol for TPT1 antibody 10824-1-AP||Download protocol|
|IP protocol for TPT1 antibody 10824-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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