TPX2 Monoclonal antibody, PBS Only

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  WB analysis using 68712-1-Ig (same clone as 68712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68712-1-Ig (same clone as 68712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68712-1-Ig (same clone as 68712-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 68712-1-Ig (same clone as 68712-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68712-1-Ig (same clone as 68712-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68712-1-Ig (same clone as 68712-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

TPX2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68712-1-PBS

Clone No.2E10C1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

2E10C1, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

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Product Information

68712-1-PBS targets TPX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33683

  Product name: Recombinant human TPX2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-395 aa of BC020207

  Sequence: MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELE

• Full Name: TPX2, microtubule-associated, homolog (Xenopus laevis)
• Calculated Molecular Weight: 747 aa, 86 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC020207
• Gene Symbol: TPX2
• Gene ID (NCBI): 22974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULW0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).