TRA2B Polyclonal antibody

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  WB analysis using 29627-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 29627-1-AP (TRA2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29627-1-AP (TRA2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 29627-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29627-1-AP (TRA2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TRA2B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29627-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Htra2 beta, hTRA2-beta, SFRS10, Splicing factor, arginine/serine-rich 10, SRFS10

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

29627-1-AP targets TRA2B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30637

  Product name: Recombinant human TRA2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-50 aa of BC005898

  Sequence: MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKS

• Full Name: transformer 2 beta homolog (Drosophila)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC005898
• Gene Symbol: TRA2B
• Gene ID (NCBI): 6434
• RRID: AB_2918335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62995
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transformer-2 protein homolog beta, also known as TRA2B, is a sequence-specific RNA-binding protein that participates in the control of pre-mRNA splicing. TRA2B can alter pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Biomed Environ Sci; Tra2β Enhances Cell Proliferation by Inducing the Expression of Transcription Factor SP1 in Cervical Cancer; Authors - Mo Juan Li ; KD Validated