TRAF1 Polyclonal antibody

TRAF1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 11543-1-AP

Synonyms

EBI6, TRAF1



Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

11543-1-AP targets TRAF1 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAF1 fusion protein Ag2131
Full Name TNF receptor-associated factor 1
Calculated molecular weight 416 aa, 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC024145
Gene symbol TRAF1
Gene ID (NCBI) 7185
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAF1 is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. TRAF1 and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. TRAF1 is observed at 45-50 kDa in western blotting, and the additional bands of lower molecular weight (~30 kDa) may represent TRAF1 cleavage fragments, similar to the results obtained by the article (PMID:11098060).

Protocols

Product Specific Protocols
WB protocol for TRAF1 antibody 11543-1-APDownload protocol
Standard Protocols
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