• Featured Product
  • KD/KO Validated

TRAF3 Polyclonal antibody

TRAF3 Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 18099-1-AP

Synonyms

CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3



Tested Applications

Positive WB detected inHEK-293 cells, multi-cells , HeLa cells, COLO 320 cells, Raji cells
Positive IP detected inRaji cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18099-1-AP is TRAF3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse, pig, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAF3 fusion protein Ag12185
Full Name TNF receptor-associated factor 3
Calculated molecular weight 568 aa, 64 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC075087
Gene symbol TRAF3
Gene ID (NCBI) 7187
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.

Protocols

Product Specific Protocols
WB protocol for TRAF3 antibody 18099-1-APDownload protocol
IP protocol for TRAF3 antibody 18099-1-APDownload protocol
FC protocol for TRAF3 antibody 18099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Cell Death Dis

Hypothermic oxygenated perfusion inhibits HECTD3-mediated TRAF3 polyubiquitination to alleviate DCD liver ischemia-reperfusion injury.

Authors - Wei Zhou
mouse,ratWB

Cell Rep

Expression of miRNA-29 in Pancreatic β Cells Promotes Inflammation and Diabetes via TRAF3.

Authors - Yi Sun
  • KD Validated
sheepWB

Mediators Inflamm

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Authors - Zhongjia Jiang
mouseWB

J Biol Chem

TNF receptor-associated factor 3 restrains B cell receptor signaling in normal and malignant B cells.

Authors - Amy L Whillock
  • KO Validated
humanWB

Front Microbiol

TRAF3 Is Required for NF-κB Pathway Activation Mediated by HTLV Tax Proteins.

Authors - Stefania Fochi
  • KO Validated
humanWB

Front Pharmacol

Protective Effects of Dioscin against Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Oxidative Stress and Inflammation.

Authors - Hong Yao