TRAF3IP1 Polyclonal antibody

TRAF3IP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14404-1-AP

Synonyms

MIP-T3, MIPT3, Microtubule-interacting protein associated with TRAF3, Intraflagellar transport protein 54 homolog, Interleukin-13 receptor alpha 1-binding protein 1

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 14404-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14404-1-AP

  IP result of anti-TRAF3IP1 (IP:14404-1-AP, 3ug; Detection:14404-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human breast cancer using 14404-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14404-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14404-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 14404-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14404-1-AP (TRAF3IP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 14404-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 14404-1-AP (TRAF3IP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

14404-1-AP targets TRAF3IP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF3IP1 fusion protein Ag5677
• Full Name: TNF receptor-associated factor 3 interacting protein 1
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC059174
• Gene Symbol: TRAF3IP1
• Gene ID (NCBI): 26146
• RRID: AB_2878054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF3IP1, also known as MIPT3, was initially characterized through its interactions with tubulin, actin, TNFR-associated factor-3 (Traf3), IL-13R1, and DISC1. It functions as an inhibitor of IL-13-mediated phosphorylation of Stat6 and in sequestration of Traf3 and DISC1 to the cytoskeleton.

Publications

Species	Application	Title
mouse	WB,IF	Int J Mol Sci; SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking; Authors - Ewud Agborbesong