Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14404-1-AP targets TRAF3IP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5677 Product name: Recombinant human TRAF3IP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 276-626 aa of BC059174 Sequence: DRDRRRVKNGEHSWDLDREKNREHDKPEKKSASSGEMSKKLSDGTFKDSKAETETEISTRASKSLTTKTSKRRSKNSVEGDSTSDAEGDAGPAGQDKSEVPETPEIPNELSSNIRRIPRPGSARPAPPRVKRQDSMEALQMDRSGSGKTVSNVITESHNSDNEEDDQFVVEAAPQLSEMSEIEMVTAVELEEEEKHGGLVKKILETKKDYEKLQQSPKPGEKERSLFESAWKKEKDIVSKEIEKLRTSIQTLCKSALPLGKIMDYIQEDVDAMQNELQMWHSENRQHAEALQQEQRITDCAVEPLKAELAELEQLIKDQQDKICAVKANILKNEEKIQKMVYSINLTSRR Predict reactive species |
| Full Name | TNF receptor-associated factor 3 interacting protein 1 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC059174 |
| Gene Symbol | TRAF3IP1 |
| Gene ID (NCBI) | 26146 |
| RRID | AB_2878054 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDR0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRAF3IP1, also known as MIPT3, was initially characterized through its interactions with tubulin, actin, TNFR-associated factor-3 (Traf3), IL-13R1, and DISC1. It functions as an inhibitor of IL-13-mediated phosphorylation of Stat6 and in sequestration of Traf3 and DISC1 to the cytoskeleton.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
| IHC protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
| IP protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
| WB protocol for TRAF3IP1 antibody 14404-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRAF3IP1 Polyclonal antibody (catalog number 14404-1-AP) has a total of 2 citations. These appear in the journals International Journal of Molecular Sciences and eLife. The associated research fields involve ciliogenesis and primary cilium assembly, specifically examining centrosomal protein regulation, intraflagellar transport trafficking, and the hierarchical pathway for distal appendage assembly. The antibody was applied in Western blot and immunofluorescence studies across mouse and human samples.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking | ||
Elife A hierarchical pathway for assembly of the distal appendages that organize primary cilia |
