TRAF3IP1 Recombinant monoclonal antibody

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  WB analysis using 87599-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87599-1-RR (TRAF3IP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity of 87599-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87599-1-RR against Human TRAF3IP1 were performed. The affinity constant is below 1 pM.

TRAF3IP1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87599-1-RR

Clone No.252996E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IFT54, Interleukin-13 receptor alpha 1-binding protein 1, Intraflagellar transport protein 54 homolog, Microtubule-interacting protein associated with TRAF3, MIPT3

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Tested Applications

• Positive WB detected in: HEK-293 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87599-1-RR targets TRAF3IP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5677

  Product name: Recombinant human TRAF3IP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 276-626 aa of BC059174

  Sequence: DRDRRRVKNGEHSWDLDREKNREHDKPEKKSASSGEMSKKLSDGTFKDSKAETETEISTRASKSLTTKTSKRRSKNSVEGDSTSDAEGDAGPAGQDKSEVPETPEIPNELSSNIRRIPRPGSARPAPPRVKRQDSMEALQMDRSGSGKTVSNVITESHNSDNEEDDQFVVEAAPQLSEMSEIEMVTAVELEEEEKHGGLVKKILETKKDYEKLQQSPKPGEKERSLFESAWKKEKDIVSKEIEKLRTSIQTLCKSALPLGKIMDYIQEDVDAMQNELQMWHSENRQHAEALQQEQRITDCAVEPLKAELAELEQLIKDQQDKICAVKANILKNEEKIQKMVYSINLTSRR

• Full Name: TNF receptor-associated factor 3 interacting protein 1
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC059174
• Gene Symbol: TRAF3IP1
• Gene ID (NCBI): 26146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDR0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF3IP1, also known as MIPT3, was initially characterized through its interactions with tubulin, actin, TNFR-associated factor-3 (Traf3), IL-13R1, and DISC1. It functions as an inhibitor of IL-13-mediated phosphorylation of Stat6 and in sequestration of Traf3 and DISC1 to the cytoskeleton.