TRAF3IP3 Polyclonal antibody

TRAF3IP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18110-1-AP

Synonyms

T3JAM, TRAF3 interacting protein 3, TRAF3IP3

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Validation Data Gallery

• 

  WB analysis of Jurkat using 18110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 18110-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 18110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 18110-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, human colon cancer tissue, mouse thymus tissue
• Positive IHC detected in: human colon cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18110-1-AP is TRAF3IP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF3IP3 fusion protein Ag12559
• Full Name: TRAF3 interacting protein 3
• Calculated Molecular Weight: 353aa,41 kDa; 551aa,64 kDa
• Observed Molecular Weight: 64 kDa, 47 kDa, 41 kDa
• GenBank Accession Number: BC110302
• Gene Symbol: TRAF3IP3
• Gene ID (NCBI): 80342
• RRID: AB_2918057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF3 interacting protein 3 (TRAF3IP3) is an essential adapter protein containing a coiled-coil domain that affects both innate and adaptive immunity. It plays a crucial role in the regulation of thymocyte development (PMID:26195727), and it also regulates T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PMID:30115741). TRAF3IP3 responses to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PMID:31390091). TRAF3IP3 has 3 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 47 kDa and 41 kDa.