Tested Applications
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11780-1-AP targets TRAF7 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2414 Product name: Recombinant human TRAF7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 70-420 aa of BC024267 Sequence: QAALQDSMPPISTPRRSDSAISVRSLHSESSMSLRSTFSLPEEEEEPEPLVFAEQPSVKLCCQLCCSVFKDPVITTCGHTFCRRCALKSEKCPVDNVKLTVVVNNIAVAEQIGELFIHCRHGCRVAGSGKPPIFEVDPRGCPFTIKLSARKDHEGSCDYRPVRCPNNPSCPPLLRMNLEAHLKECEHIKCPHSKYGCTFIGNQDTYETHLETCRFEGLKEFLQQTDDRFHEMHVALAQKDQEIAFLRSMLGKLSEKIDQLEKSLELKFDVLDENQSKLSEDLMEFRRDASMLNDELSHINARLNMGILGSYDPQQIFKCKGTFVGHQGPVWCLCVYSMGDLLFSGSSDKTI Predict reactive species |
| Full Name | TNF receptor-associated factor 7 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 67-75 kDa |
| GenBank Accession Number | BC024267 |
| Gene Symbol | TRAF7 |
| Gene ID (NCBI) | 84231 |
| RRID | AB_2877793 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6Q0C0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAF7 antibody 11780-1-AP | Download protocol |
| IHC protocol for TRAF7 antibody 11780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TRAF7 Antibody (Cat# 11780-1-AP) has 13 total citations published in journals including Nature Communications, Kidney International, Cardiovascular Research, Journal of Experimental & Clinical Cancer Research, Cell Death Discovery, Free Radical Biology and Medicine, Molecular Therapy Nucleic Acids, and others. Research fields span cardiac hypertrophy, glioma and prostate cancer, endothelial dysfunction in diabetes, fatty liver disease, kidney development (CAKUT), viral RLR signaling, zebrafish embryogenesis, and testicular apoptosis.
| Species | Application | Title |
|---|---|---|
Kidney Int Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney. | ||
Nat Commun Hypoxia-induced conversion of sensory Schwann cells into repair cells is regulated by HDAC8 | ||
J Exp Clin Cancer Res TRAF7 knockdown induces cellular senescence and synergizes with lomustine to inhibit glioma progression and recurrence | ||
Cardiovasc Res Cardiac tumour necrosis factor receptor-associated factor 7 mediates the ubiquitination of apoptosis signal-regulating kinase 1 and aggravates cardiac hypertrophy | ||
Cell Death Discov TRAF7-targeted HOXA5 acts as a tumor suppressor in prostate cancer progression and stemness via transcriptionally activating SPRY2 and regulating MEK/ERK signaling | ||
Free Radic Biol Med Hydrogen sulfide improves endothelial barrier function by modulating the ubiquitination degradation of KLF4 through TRAF7 S-sulfhydration in diabetic aorta |
