TRAF7 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRAF7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11780-1-AP

Synonyms

RFWD1, RING finger protein 119, RNF119, TRAF7

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Validation Data Gallery

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  WB analysis of mouse heart using 11780-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11780-1-AP (TRAF7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11780-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11780-1-AP (TRAF7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11780-1-AP

  IP result of anti-TRAF7 (IP:11780-1-AP, 3ug; Detection:11780-1-AP 1:300) with mouse liver tissue lysate 3000ug.

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  IHC staining of human colon cancer using 11780-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11780-1-AP (TRAF7 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

11780-1-AP targets TRAF7 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF7 fusion protein Ag2414
• Full Name: TNF receptor-associated factor 7
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 67-75 kDa
• GenBank Accession Number: BC024267
• Gene Symbol: TRAF7
• Gene ID (NCBI): 84231
• RRID: AB_2877793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse, human	IF	Kidney Int; Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.; Authors - Rik Westland
human	WB	Mol Ther Nucleic Acids; HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.; Authors - Zhi Zhang
human	WB	Virol Sin; TRAF7 negatively regulates the RLR signaling pathway by facilitating the K48-linked ubiquitination of TBK1; Authors - Jing-Ping Huang ; KO Validated
human	WB,CoIP	Cell Death Discov; TRAF7-targeted HOXA5 acts as a tumor suppressor in prostate cancer progression and stemness via transcriptionally activating SPRY2 and regulating MEK/ERK signaling; Authors - Jianfeng Ye
mouse,rat	WB	Free Radic Biol Med; Hydrogen sulfide improves endothelial barrier function by modulating the ubiquitination degradation of KLF4 through TRAF7 S-sulfhydration in diabetic aorta; Authors - Qianzhu Li
human	IF	Genes (Basel); Expression of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) Candidate Genes EDA2R, PCDH9, and TRAF7 in Normal Human Kidney Development and CAKUT; Authors - Jelena Kelam