TRAFD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRAFD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27741-1-AP

Synonyms

TRAF-type zinc finger domain-containing protein 1, Protein FLN29, FLN29

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Validation Data Gallery

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  WB analysis of HEK-293 using 27741-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27741-1-AP

  WB result of TRAFD1 antibody (27741-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAFD1 transfected A549 cells.

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  WB analysis of K-562 using 27741-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27741-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 27741-1-AP

  IP result of anti-TRAFD1 (IP:27741-1-AP, 4ug; Detection:27741-1-AP 1:800) with HEK-293 cells lysate 1575 ug.

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  IHC staining of human tonsillitis using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27741-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, A549 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human tonsillitis tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27741-1-AP targets TRAFD1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAFD1 fusion protein Ag26945
• Full Name: TRAF-type zinc finger domain containing 1
• Calculated Molecular Weight: 582 aa, 65 kDa
• Observed Molecular Weight: 65-80 kDa
• GenBank Accession Number: BC003553
• Gene Symbol: TRAFD1
• Gene ID (NCBI): 10906
• RRID: AB_2880958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAFD1, also known as TRAF-type zinc finger domain containing 1, is a protein that plays a significant role in immune system signaling pathways. It was first identified as an interferon- (IFN) and lipopolysaccharide- (LPS) inducible factor. TRAFD1 contains a TRAF-type zinc finger domain at its N-terminus and a TRAF6-binding motif in its middle region, which allows it to interact with other TRAF proteins and modulate immune responses. In the context of immune signaling, TRAFD1 has been shown to suppress the inflammatory responses to innate immunity by inhibiting Toll-like receptor 4 (TLR4) dependent NF-κB and MAPK activation in monocytes/macrophages. This suggests that TRAFD1 can act as a negative regulator of immune signaling, dampening the inflammatory response. Moreover, TRAFD1 has been implicated in human diseases. It is overexpressed in many B cell-related cancers, and single nucleotide polymorphisms (SNPs) in TRAFD1 have been linked to non-Hodgkin's lymphoma.