TRAIL/CD253 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 84280-1-RR (same clone as 84280-1-PBS)

  Human milk samples were subjected to SDS PAGE followed by western blot with 84280-1-RR (TRAIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84280-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01166-1

  Sandwich ELISA standard curve of MP01166-1, Human TRAIL Recombinant Matched Antibody Pair - PBS only. 84280-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02339. 84280-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL

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  Sample test of MP01166-1

  Saliva of six individual healthy human donors was measured. The human TRAIL concentration of detected samples was determined to be 809.9 pg/mL with a range of 159.6 - 1,340.0 pg/mL.

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  Sample test of MP01166-1

  Serum of six individual healthy human donors was measured. The human TRAIL concentration of detected samples was determined to be 182.8 pg/mL with a range of 33.7 - 368.3 pg/mL.

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  Sample test of MP01166-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean TRAIL concentration was undetectable in unstimulated PBMC supernatant, 22.3 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean TRAIL concentration was determined to be 1.0 pg/mL in unstimulated PBMC supernatant, 22.9 pg/mL in PHA stimulated PBMC supernatant.

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  Cytometric bead array standard curve of MP01166-1

  Cytometric bead array standard curve of MP01166-1, TRAIL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84280-2-PBS. Detection antibody: 84280-1-PBS. Standard: RP02339. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84280-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 84280-1-PBS against Human TRAIL were performed. The affinity constant is 0.43 nM.

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  Affinity and Kinetic Characterization of 84280-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84280-1-RR against Human TRAIL were performed. The affinity constant is 0.461 nM.

TRAIL/CD253 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84280-1-PBS

Clone No.241419C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TNFSF10, TRAIL, 241419C12, Apo-2 ligand, CD253

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Product Information

84280-1-PBS targets TRAIL/CD253 as part of a matched antibody pair:

MP01166-1: 84280-2-PBS capture and 84280-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50591
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.