TRAK2 Polyclonal antibody

TRAK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13770-1-AP

Synonyms

Trafficking kinesin-binding protein 2, KIAA0549, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein, ALS2CR3

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Validation Data Gallery

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  WB analysis of human liver using 13770-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13770-1-AP (TRAK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13770-1-AP

  IP result of anti-TRAK2 (IP:13770-1-AP, 4ug; Detection:13770-1-AP 1:500) with HepG2 cells lysate 4000ug.

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  IHC staining of human liver cancer using 13770-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13770-1-AP (TRAK2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 13770-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13770-1-AP (TRAK2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 13770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAK2 antibody (13770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13770-1-AP

  Immunofluorescent analysis of Hela cells, using TRAK2 antibody 13770-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 13770-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13770-1-AP (TRAK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

13770-1-AP targets TRAK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAK2 fusion protein Ag4753
• Full Name: trafficking protein, kinesin binding 2
• Calculated Molecular Weight: 914 aa, 101 kDa
• Observed Molecular Weight: 101-106 kDa
• GenBank Accession Number: BC040668
• Gene Symbol: TRAK2
• Gene ID (NCBI): 66008
• RRID: AB_2207961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Trafficking kinesin-binding protein 2 (TRAK2) is a member of the TRAK family of kinesin adaptor proteins. In mammals, there are two members of the TRAK family, TRAK1 and TRAK2 (PMID: 25653102). TRAK1 and TRAK2 play roles in lysosomal and mitochondrial trafficking in cells. They function as kinesin adaptors linking kinesin heavy chain (KHC) to mitochondria (PMID: 28364549). TRAK2 has been found to act as a trafficking factor for the K+ channel Kir2.1 and γ-Aminobutyric acid type A (GABAA) receptor (PMID: 16895905; 24122114).

Publications

Species	Application	Title
human	WB	FASEB J; Adaptation of mitochondrial network dynamics and velocity of mitochondrial movement to chronic stress present in fibroblasts derived from patients with sporadic form of Alzheimer's disease.; Authors - Karolina Drabik
rat	WB	J Cell Sci; Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19.; Authors - Katarzyna Majstrowicz
mouse	WB	Exp Cell Res; Antagomirs targeting miR-142-5p attenuate pilocarpine-induced status epilepticus in mice.; Authors - Haifeng Zhang
rat	WB	Bioeng Transl Med; Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36; Authors - Zhijie Zhu