TRAPPC1 Polyclonal antibody

TRAPPC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19598-1-AP

Synonyms

BET5, BET5 homolog, Multiple myeloma protein 2, MUM 2, MUM2, TRAPPC1

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Validation Data Gallery

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  WB analysis using 19598-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19598-1-AP (TRAPPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19598-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19598-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19598-1-AP targets TRAPPC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAPPC1 fusion protein Ag5608
• Full Name: trafficking protein particle complex 1
• Calculated Molecular Weight: 145 aa, 17 kDa
• Observed Molecular Weight: 14-17 kDa
• GenBank Accession Number: BC032717
• Gene Symbol: TRAPPC1
• Gene ID (NCBI): 58485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC1, also known as BET5 or MUM2, is a part of the multisubunit TRAPP (transport protein particle) complexes which are involved in the tethering process at different trafficking steps of vesicle transport (PMID: 16828797). TRAPPC1 may play a role in vesicular transport from the endoplasmic reticulum to Golgi (PMID: 10582700).