TRAPPC12 Polyclonal antibody, PBS Only

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• TRAPPC12 Polyclonal antibody, PBS Only (24555-1-PBS)

• 

  WB analysis using 24555-1-AP (same clone as 24555-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

  WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

  IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TRAPPC12 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 24555-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

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Product Information

24555-1-PBS targets TRAPPC12 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18332

  Product name: Recombinant human TTC15 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 395-735 aa of BC014164

  Sequence: AAVDLCGRLLTAHGQGYGKSGLLTSHTTDSLQLWFVRLALLVKLGLFQNAEMEFEPFGNLDQPDLYYEYYPHVYPGRRGSMVPFSMRILHAELQQYLGNPQESLDRLHKVKTVCSKILANLEQGLAEDGGMSSVTQEGRQASIRLWRSRLGRVMYSMANCLLLMKDYVLAVDAYHSVIKYYPEQEPQLLSGIGRISLQIGDIKTAEKYFQDVEKVTQKLDGLQGKIMVLMNSAFLHLGQNNFAEAHRFFTEILRMDPRNAVANNNAAVCLLYLGKLKDSLRQLEAMVQQDPRHYLHESVLFNLTTMYELESSRSMQKKQALLEAVAGKEGDSFNTQCLKLA

• Full Name: tetratricopeptide repeat domain 15
• Calculated Molecular Weight: 735 aa, 79 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC014164
• Gene Symbol: TRAPPC12
• Gene ID (NCBI): 51112
• RRID: AB_3742117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVT3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).