Tested Applications
| Positive WB detected in | HL-60 cells, MCF-7 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human colon cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12074-1-AP targets TRAPPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2737 Product name: Recombinant human TRAPPC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC010866 Sequence: MAIFSVYVVNKAGGLIYQLDSYAPRAEAEKTFSYPLDLLLKLHDERVLVAFGQRDGIRVGHAVLAINGMDVNGRYTADGKEVLEYLGNPANYPVSIRFGRPRLTSNEKLMLASMFHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADPRQAGIDSLLRKIYEIYSDFALKNPFYSLEMPIRCELFDQNLKLALEVAEKAGTFGPGS Predict reactive species |
| Full Name | trafficking protein particle complex 4 |
| Calculated Molecular Weight | 219 aa, 24 kDa |
| Observed Molecular Weight | 24-27 kDa |
| GenBank Accession Number | BC010866 |
| Gene Symbol | TRAPPC4 |
| Gene ID (NCBI) | 51399 |
| RRID | AB_2208147 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y296 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAPPC4 antibody 12074-1-AP | Download protocol |
| IHC protocol for TRAPPC4 antibody 12074-1-AP | Download protocol |
| IP protocol for TRAPPC4 antibody 12074-1-AP | Download protocol |
| WB protocol for TRAPPC4 antibody 12074-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRAPPC4 Polyclonal antibody (Cat# 12074-1-AP) has 1 citation to date. It was cited in Cancer Research (IF 22.6), a study investigating how TRAPPC4 promotes GPX4 stability to drive ferroptosis resistance and tumor progression in head and neck squamous cell carcinoma. The associated research fields include ferroptosis regulation, cancer biology, and head and neck oncology.
| Species | Application | Title |
|---|---|---|
Cancer Res TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma |
