Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, mouse brain tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16014-1-AP targets TRAPPC9, NIBP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8791 Product name: Recombinant human TRAPPC9,NIBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 563-912 aa of BC006206 Sequence: SCQENLLQDLSDDGISVSGFPLSSPFRQVVRPRVEGKPVNPPESNKAGDYSHVKTLEAVLNFKYSGGPGHTEGYYRNLSLGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNSTEHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPESPGEKGQFANPKQLEEERREARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLVDGQPCDREAVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSSTFYLDAVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEAQA Predict reactive species |
| Full Name | trafficking protein particle complex 9 |
| Calculated Molecular Weight | 139 kDa |
| Observed Molecular Weight | 128-140 kDa |
| GenBank Accession Number | BC006206 |
| Gene Symbol | NIBP/Trappc9 |
| Gene ID (NCBI) | 83696 |
| RRID | AB_2256482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96Q05 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRAPPC9, also named KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cell differentiation and play a role in vesicular transport from the endoplasmic reticulum to the Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter. Western blotting indicates the molecular weight of TRAPPC9 is 130-140 kDa, and 250 kDa may be detected with extensive posttranslational modification of TRAPPC9.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAPPC9, NIBP antibody 16014-1-AP | Download protocol |
| IHC protocol for TRAPPC9, NIBP antibody 16014-1-AP | Download protocol |
| IP protocol for TRAPPC9, NIBP antibody 16014-1-AP | Download protocol |
| WB protocol for TRAPPC9, NIBP antibody 16014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-NIBP (TRAPPC9) antibody (Cat# 16014-1-AP) has 13 citations published in journals including Signal Transduction and Targeted Therapy, Cancer Cell, Nature Communications, PNAS, EMBO Journal, EMBO Reports, Int J Biol Sci, J Biol Chem, Oncol Rep, Tumour Biol, J Cell Biochem, PLoS ONE, and Function. Research fields span NF-κB signaling, cancer metastasis, ciliogenesis, secretory pathway trafficking, stress granule assembly, myeloid progenitor differentiation, ubiquitination, and osteoclast biology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther RING finger 138 deregulation distorts NF-кB signaling and facilities colitis switch to aggressive malignancy.
| ||
Cancer Cell Lipid oxidation reprogramming in cancer-associated fibroblasts enhances CD8(+) T cell cytotoxicity and therapeutic response. | ||
Nat Commun Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2 | ||
Int J Biol Sci Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice | ||
Proc Natl Acad Sci U S A Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome. | ||
Reviews
What customers say
One reviewer (Gaurav Kumar) rated this product 5/5 stars. They used it for Western Blot and Immunoprecipitation at a 1:1000 dilution on HEK293 cells and reported that it "works in Co-IP." Overall, the single review reflects a positive experience with the antibody performing well in co-immunoprecipitation applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (02-27-2026) | Works in Co-IP
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