TRAV20 Polyclonal antibody

TRAV20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13132-1-AP

Synonyms

TCRAV20S1, TCRAV30S1, TRAV20

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Validation Data Gallery

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  WB analysis of K-562 using 13132-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13132-1-AP (TRAV20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 13132-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13132-1-AP (TRAV20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 13132-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13132-1-AP (TRAV20 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 13132-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13132-1-AP (TRAV20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13132-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13132-1-AP (TRAV20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13132-1-AP targets TRAV20 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAV20 fusion protein Ag3841
• Full Name: T cell receptor alpha variable 20
• Calculated Molecular Weight: 273 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC025727
• Gene Symbol: TRAV20
• Gene ID (NCBI): 28663
• RRID: AB_1211619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.