TRBP Monoclonal antibody

TRBP Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1C9A10

Cat no : 66792-1-Ig

Synonyms

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66792-1-Ig targets TRBP in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRBP fusion protein Ag8269
Full Name TAR (HIV-1) RNA binding protein 2
Calculated molecular weight 39 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC005860
Gene symbol TARBP2
Gene ID (NCBI) 6895
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43–46 kDa, are expressed in human cells. (PMID: 16142218)

Protocols

Product Specific Protocols
WB protocol for TRBP antibody 66792-1-IgDownload protocol
IHC protocol for TRBP antibody 66792-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols