TRBV5-4 Polyclonal antibody

TRBV5-4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13524-1-AP

Synonyms

TCRBV5S4, TCRBV5S6A3N2T, TRBV5 4, TRBV54, TRBV5-4

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Validation Data Gallery

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  WB analysis of U-937 using 13524-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13524-1-AP (TRBV5-4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 13524-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13524-1-AP (TRBV5-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13524-1-AP targets TRBV5-4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRBV5-4 fusion protein Ag4337
• Full Name: T cell receptor beta variable 5-4
• Calculated Molecular Weight: 308 aa, 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC028083
• Gene Symbol: TRBV5-4
• Gene ID (NCBI): 28611
• RRID: AB_1167555
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.