Anti-Human TREM-1/CD354 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98154-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TREM-1/CD354 Rabbit Recombinant Antibody (98154-1-RR, Clone: 241279D10) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98154-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TREM-1/CD354 Rabbit Recombinant Antibody (98154-1-RR, Clone: 241279D10)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98154-1-RR against Human TREM-1/CD354 were performed. The affinity constant is 0.676 nM.

TREM-1/CD354 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98154-1-RR

Clone No.241279D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TREM1, TREM 1, CD354, 241279D10

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98154-1-RR targets TREM-1/CD354 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: triggering receptor expressed on myeloid cells 1
• Calculated Molecular Weight: 26kDa
• GenBank Accession Number: NM_018643.5
• Gene Symbol: TREM1
• Gene ID (NCBI): 54210
• RRID: AB_3672297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NP99-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Triggering receptor expressed on myeloid cells 1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.