TREM-1/CD354 Polyclonal antibody

• 

  WB analysis of human peripheral blood leukocyte using 11791-1-AP

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:450 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11791-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11791-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11791-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11791-1-AP (TREM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 11791-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11791-1-AP (TREM-1/CD354 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11791-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11791-1-AP (TREM-1/CD354 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TREM-1/CD354 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11791-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

TREM1, CD354, TREM 1

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Tested Applications

• Positive WB detected in: human peripheral blood leukocyte, human liver tissue, human spleen tissue, mouse lung tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 13 publications below
• CoIP: See 1 publications below

Product Information

11791-1-AP targets TREM-1/CD354 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2418

  Product name: Recombinant human TREM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-234 aa of BC017773

  Sequence: MRKTRLWGLLWMLFVSELRAATKLTEEKYELKEGQTLDVKCDYTLEKFASSQKAWQIIRDGEMPKTLACTERPSKNSHPVQVGRIILEDYHDHGLLRVRMVNLQVEDSGLYQCVIYQPPKEPHMLFDRIRLVVTKGFSGTPGSNENSTQNVYKIPPTTTKALCPLYTSPRTVTQAPPKSTADVSTPDSEINLTNVTDIIRVPVFNIVILLAGGFLSKSLVFSVLFAVTLRSFVP

• Full Name: triggering receptor expressed on myeloid cells 1
• Calculated Molecular Weight: 234 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC017773
• Gene Symbol: TREM1
• Gene ID (NCBI): 54210
• RRID: AB_2208556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NP99
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Triggering receptor expressed on myeloid cells 1 (TREM1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Theranostics; A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation.; Authors - Hui-Hui Yang
mouse	WB,IF	Int Immunopharmacol; Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages; Authors - Junhao Wan
	WB,IF	ACS Chem Neurosci; Pilot Screening of TREM1 Inhibitors in Cell-Based Reporter Assays Reflecting TREM1/DAP12 Receptor Assembly and Functionality; Authors - Natalia Filippova
mouse	WB	Int Immunopharmacol; Activation of NLRP3 inflammasome up-regulates TREM-1 expression in murine macrophages via HMGB1 and IL-18.; Authors - Wen-Jing Zhong
mouse	IF	Acta Trop; Malaria-derived exosomes exacerbate liver injury during blood stage of Plasmodium berghei infection; Authors - Xin Zhang
human	WB,IHC	J Inflamm Res; Overexpression of TREM1 is Associated with the Immune-Suppressive Microenvironment and Unfavorable Prognosis in Pan-Cancer; Authors - Xinwei Zhou