TREM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TREM2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 13483-1-AP

Synonyms

TREM 2, TREM2, Trem2a, Trem2b, Trem2c

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Validation Data Gallery

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  IHC staining of mouse brain using 13483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13483-1-AP (TREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13483-1-AP (TREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Product Information

13483-1-AP targets TREM2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TREM2 fusion protein Ag4299
• Full Name: triggering receptor expressed on myeloid cells 2
• Calculated Molecular Weight: 222 aa, 25 kDa
• GenBank Accession Number: BC032362
• Gene Symbol: TREM2
• Gene ID (NCBI): 54209
• RRID: AB_2877955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).

Publications

Species	Application	Title
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	WB	Arterioscler Thromb Vasc Biol; Sexually Dimorphic Relationships Among Saa3 (Serum Amyloid A3) Inflammation, and Cholesterol Metabolism Modulate Atherosclerosis in Mice.; Authors - Alan Chait
mouse	IF	J Neuroinflammation; Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway.; Authors - Ruozhi Dang
mouse	WB,IF	Chem Biol Interact; Harpagide alleviate neuronal apoptosis and blood-brain barrier leakage by inhibiting TLR4/MyD88/NF-κB signaling pathway in Angiotensin II-induced microglial activation in vitro.; Authors - Yunwei Lu
mouse	WB,IF	Toxicol Appl Pharmacol; Methamphetamine-mediated dissemination of β-amyloid: Disturbances in endocytosis, transport and clearance of β-amyloid in microglial BV2 cells.; Authors - Liyuan Tao
mouse	IF,WB	Neural Plast; Microglia TREM2: A Potential Role in the Mechanism of Action of Electroacupuncture in an Alzheimer's Disease Animal Model.; Authors - Yujie Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hongxue (Verified Customer) (01-12-2021)

Works for IF staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100

TREM2 Antibody Immunofluorescence validation (1:100 dilution) in (Cat no:13483-1-AP)