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Tested Applications
| Positive WB detected in | MCF7 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
20062-1-AP targets TRERF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | transcriptional regulating factor 1 |
| Calculated Molecular Weight | 132 kDa |
| Observed Molecular Weight | 132-140 kDa |
| GenBank Accession Number | NM_033502 |
| Gene Symbol | TRERF1 |
| Gene ID (NCBI) | 55809 |
| RRID | AB_10666203 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRERF1, also named as BCAR2, RAPA and TREP132, activates transcription of CYP11A1. TRERF1 interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. The antibody recognizes the C-term of TRERF1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRERF1 antibody 20062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
