TRERF1 Polyclonal antibody

TRERF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20062-1-AP

Synonyms

HSA277276, RAPA, RP1 139D8.5, TReP 132, TREP132, TRERF1, Zinc finger protein rapa

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Validation Data Gallery

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  WB analysis of MCF-7 using 20062-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20062-1-AP (TRERF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20062-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20062-1-AP (TRERF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF7 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

20062-1-AP targets TRERF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transcriptional regulating factor 1
• Calculated Molecular Weight: 132 kDa
• Observed Molecular Weight: 132-140 kDa
• GenBank Accession Number: NM_033502
• Gene Symbol: TRERF1
• Gene ID (NCBI): 55809
• RRID: AB_10666203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRERF1, also named as BCAR2, RAPA and TREP132, activates transcription of CYP11A1. TRERF1 interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. The antibody recognizes the C-term of TRERF1.

Publications

Species	Application	Title
human	IHC	Cancer Manag Res; MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression.; Authors - Jianlong Wang