Tested Applications
| Positive WB detected in | MCF7 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20062-1-AP targets TRERF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | transcriptional regulating factor 1 |
| Calculated Molecular Weight | 132 kDa |
| Observed Molecular Weight | 132-140 kDa |
| GenBank Accession Number | NM_033502 |
| Gene Symbol | TRERF1 |
| Gene ID (NCBI) | 55809 |
| RRID | AB_10666203 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRERF1, also named as BCAR2, RAPA and TREP132, activates transcription of CYP11A1. TRERF1 interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. The antibody recognizes the C-term of TRERF1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRERF1 antibody 20062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRERF1 Polyclonal antibody (Catalog No. 20062-1-AP) has a total of 1 citation. It appears in the journal Cancer Management Research (2022), in a study titled "MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression." The associated research field is oncology, specifically colorectal cancer, where the antibody was applied in immunohistochemistry (IHC) on human samples.
| Species | Application | Title |
|---|---|---|
Cancer Manag Res MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression. |



