Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24876-1-AP targets TREX1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18325 Product name: Recombinant human TREX1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-369 aa of BC023630 Sequence: MGPGARRQGRIVQGRPEMCFCPPPTPLPPLRILTLGTHTPTPCSSPGSAAGTYPTMGSQALPPGPMQTLIFFDMEATGLPFSQPKVTELCLLAVHRCALESPPTSQGPPPTVPPPPRVVDKLSLCVAPGKACSPAASEITGLSTAVLAAHGRQCFDDNLANLLLAFLRRQPQPWCLVAHNGDRYDFPLLQAELAMLGLTSALDGAFCVDSITALKALERASSPSEHGPRKSYSLGSIYTRLYGQSPPDSHTAEGDVLALLSICQWRPQALLRWVDAHARPFGTIRPMYGVTASARTKPRPSAVTTTAHLATTRNTSPSLGESRGTKDLPPVKDPGALSREGLLAPLGLLAILTLAVATLYGLSLATPGE Predict reactive species |
| Full Name | three prime repair exonuclease 1 |
| Calculated Molecular Weight | 369 aa, 39 kDa |
| Observed Molecular Weight | 32-39 kDa |
| GenBank Accession Number | BC023630 |
| Gene Symbol | TREX1 |
| Gene ID (NCBI) | 11277 |
| RRID | AB_2879772 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NSU2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TREX1 (three prime repair exonuclease 1), also known as trophoblast expressed 1, CRV, AGS1, AGS5, DRN3, HERNS or DNase III, is a member of the exonuclease superfamily and belongs to the TREX family. TREX1 may play a role in DNA repair. TREX1 is expressed in thymus, spleen, liver, brain, heart, small intestine and colon. Mutations or defects in the gene encoding TREX1 have been associated with a variety of diseases, including systemic lupus erythematosus, chilblain lupus (CHBL), Aicardi-Goutieres syndrome type 1 (AGS1) and type 5 (AGS5).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TREX1 antibody 24876-1-AP | Download protocol |
| IP protocol for TREX1 antibody 24876-1-AP | Download protocol |
| WB protocol for TREX1 antibody 24876-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Cell Host Microbe Pathogen-driven CRISPR screens identify TREX1 as a regulator of DNA self-sensing during influenza virus infection | ||
Free Radic Biol Med S-nitrosation in endothelium: different outcomes of NRF2 and GSNOR loss. | ||
Cell Rep Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition | ||
Front Immunol Two rare mutations in homozygosity synergize to silence TREX1 in Aicardi-Goutières syndrome | ||
J Ethnopharmacol Buyang Huanwu Decoction alleviates vascular cognitive impairment by inhibiting neuroinflammation via regulation of the p53/cGAS/STING pathway. | ||
Int J Mol Sci Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells |
Reviews
What customers say
The single review rates this antibody 4 out of 5 for Western Blot. The user confirmed it recognizes the specific TREX1 band, validated by knockout. They noted mild background and found the signal weak at 1:1000 dilution but improved at 1:500. The antibody performed well across human and mouse cell lines, including MEFs, HeLa, HEK293FT, and RPE1. Overall, the reviewer found it reliable for detecting the target with appropriate dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chantal (Verified Customer) (10-04-2022) | reconignises the specific band, validated by KO of TREX1. A little bit of background. the signal is weak at 1:1000 dilution, better at 1:500. Works on human and mouse cell lines.
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