TRH Polyclonal antibody, PBS Only

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• TRH Polyclonal antibody, PBS Only (25332-1-PBS)

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  WB analysis using 25332-1-AP (same clone as 25332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25332-1-AP (TRH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25332-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 25332-1-AP (same clone as 25332-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25332-1-AP (TRH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25332-1-PBS in a different storage buffer formulation.

TRH Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 25332-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Prothyroliberin, Pro-thyrotropin-releasing hormone, Protirelin, Thyrotropin-releasing hormone, TRF

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Product Information

25332-1-PBS targets TRH in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12623

  Product name: Recombinant human TRH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-242 aa of BC074889

  Sequence: MPGPWLLLALALTLNLTGVPGGRAQPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHSASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRRSPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLLGLLDDLSRSQGAEEKRQHPGRRAAWVREPLEE

• Full Name: thyrotropin-releasing hormone
• Calculated Molecular Weight: 242 aa, 27 kDa
• Observed Molecular Weight: 27-33 kDa
• GenBank Accession Number: BC074889
• Gene Symbol: TRH
• Gene ID (NCBI): 7200
• RRID: AB_3085786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20396
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978).