TRIB1 Polyclonal antibody

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  WB analysis using 27899-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 27899-1-AP (TRIB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 27899-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27899-1-AP (TRIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27899-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27899-1-AP (TRIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRIB1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27899-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C8FW, GIG 2, GIG2, GIG-2, G-protein-coupled receptor-induced gene 2 protein

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27899-1-AP targets TRIB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27541

  Product name: Recombinant human TRIB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-150 aa of BC063292

  Sequence: MRVGPVRSAMSGASQPRGPALLFPATRGVPAKRLLDADDAAAVAAKCPRLSECSSPPDYLSPPGSPCSPQPPPAAPGAGGGSGSAPGPSRIADYLLLPLAEREHVSRALCIHTGRELRCKVFPIKHYQDKIRPYIQLPSHSNITGIVEVI

• Full Name: tribbles homolog 1 (Drosophila)
• Calculated Molecular Weight: 372 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC063292
• Gene Symbol: TRIB1
• Gene ID (NCBI): 10221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RU8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIB1, a pseudokinase encoded by the tribbles homolog 1 gene, regulates hepatic lipogenesis and lipid metabolism by modulating C/EBPα degradation, thereby influencing plasma lipid profiles and atherosclerotic risk. It is expressed in macrophages and vascular smooth muscle cells, linking metabolic and inflammatory pathways in cardiometabolic disorders (PMID: 26517927, PMID: 40331689).