TRIM11 Polyclonal antibody

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TRIM11 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10851-1-AP Copied!

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

BIA1, E3 ubiquitin-protein ligase TRIM11, EC:2.3.2.27, Protein BIA1, RING finger protein 92

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  WB analysis using 10851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10851-1-AP (TRIM11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HeLa using 10851-1-AP

  IP result of anti-TRIM11 (IP:10851-1-AP, 4ug; Detection:10851-1-AP 1:1000) with HeLa cells lysate 1080 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human colon cancer using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human colon cancer using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat heart using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat lung using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat stomach using 10851-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10851-1-AP (TRIM11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, mouse stomach tissue, rat heart tissue, mouse lung tissue, human stomach cancer tissue, human tonsillitis tissue, rat lung tissue, rat stomach tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10851-1-AP targets TRIM11 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1308

  Product name: Recombinant human TRIM11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-202 aa of BC011629

  Sequence: MELRTVCRVPGLVETLRRFRGDVTLDPDTANPELILSEDRRSVQRGDLRQALPDSPERFDPGPCVLGQERFTSGRHYWEVEVGDRTSWALGVCRENVNRKEKGELSAGNGFWILVFLGSYYNSSERALAPLRDPPRRVGIFLDYEAGHLSFYSATDGSLLFIFPEIPFSGTLRPLFSPLSSSPTPMTICRPKGGSGDTLAPQ

• Full Name: tripartite motif-containing 11
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC011629
• Gene Symbol: TRIM11
• Gene ID (NCBI): 81559
• RRID: AB_2209210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96F44
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM11(Tripartite motif-containing protein 11) is also named as RNF92 and Belongs to the TRIM/RBCC family. It is a member of the tripartite-motif-containing protein family and is known to destabilize humanin, an inhibitor of Alzheimer-like neuronal insults(PMID: 16904669). This protein has 3 isoforms produced by alternative splicing.

Publications

What published studies show

Anti-TRIM11 antibody (Cat# 10851-1-AP) has 16 citations spanning journals including Science, Signal Transduction and Targeted Therapy, Oncogene, FASEB J, Int J Biol Sci, J Hazard Mater, Neoplasia, Cancer Cell Int, Funct Integr Genomics, Pathol Res Pract, Cell Biol Int, Bioengineered, Naunyn Schmiedebergs Arch Pharmacol, Biomed Res Int, Int J Gen Med, and Hum Genome Var. Research fields cover oncology (glioma, breast, thyroid, ovarian, lung, esophageal, cholangiocarcinoma, osteosarcoma), neurodegeneration, cardioprotection, pulmonary fibrosis, diabetic nephropathy, and ferroptosis.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Signal Transduct Target Ther; Nucleosome assembly protein-like 1 degradation-dependent novel cardioprotection mechanism of Wnt2 against ischemia‒reperfusion injury; Authors - Ying Wang
		Science; TRIM11 protects against tauopathies and is down-regulated in Alzheimer's disease; Authors - Zi-Yang Zhang
human	WB,IF,CoIP	Int J Biol Sci; SOX13/TRIM11/YAP axis promotes the proliferation, migration and chemoresistance of anaplastic thyroid cancer.; Authors - Jianing Tang
mouse	WB,IF,CoIP	J Hazard Mater; The proteasome-dependent degradation of ALKBH5 regulates ECM deposition in PM2.5 exposure-induced pulmonary fibrosis of mice.; Authors - Yaling Zhang
human	IHC,IF	Oncogene; TRIM11 is overexpressed in high-grade gliomas and promotes proliferation, invasion, migration and glial tumor growth.; Authors - Di K K
		Cancer Cell Int; USP8 promotes the tumorigenesis of intrahepatic cholangiocarcinoma via stabilizing OGT; Authors - Guo Long