Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells, mouse spleen tissue, mouse thymus tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13663-1-AP targets TRIM17 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4572 Product name: Recombinant human TRIM17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 177-477 aa of BC033788 Sequence: KVKERRERIVLEFEKMNLYLVEEEQRLLQALETEEEETASRLRESVACLDRQGHSLELLLLQLEERSTQGPLQMLQDMKEPLSRKNNVSVQCPEVAPPTRPRTVCRVPGQIEVLRGFLEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPFFCLGAPKSGQMVISTVTMWVKG Predict reactive species |
| Full Name | tripartite motif-containing 17 |
| Calculated Molecular Weight | 477 aa, 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC033788 |
| Gene Symbol | TRIM17 |
| Gene ID (NCBI) | 51127 |
| RRID | AB_10644443 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y577 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM17 (Tripartite motif-containing 17) also known as RNF16 (Ring Finger protein 16) or terf (testis RING finger protein), is a member of the TRIM family, a large class of RING-containing E3 ubiquitin-ligases. It is expressed at low levels in adult tissues, except in testis and in some brain regions (PMID: 34069831). TRIM17 contributes to gastric cancer survival through regulating BAX protein stability and antagonizing apoptosis, which provides a promising therapeutic target for GC treatment and a biomarker for prognosis (PMID: 27562068, 37697039).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM17 antibody 13663-1-AP | Download protocol |
| IHC protocol for TRIM17 antibody 13663-1-AP | Download protocol |
| WB protocol for TRIM17 antibody 13663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-TRIM17 (Cat# 13663-1-AP) has 5 citations published in Cell Death & Differentiation, Cell Death & Disease, Journal of Cellular and Molecular Medicine, and Journal of Biological Chemistry. The research spans cancer biology—including prostate, gastric, colorectal, hepatocellular carcinoma, and glioblastoma—and covers topics such as apoptosis regulation, ferroptosis resistance, ubiquitin-mediated protein degradation, and drug resistance mechanisms.
| Species | Application | Title |
|---|---|---|
Cell Death Differ TRIM17 downregulation modulates CRPC progression and enzalutamide resistance by derepressing BCL2 expression via the p53-dependent and p53-independent pathways. | ||
Cell Death Differ The E3 ubiquitin ligase TRIM17 promotes gastric cancer survival and progression via controlling BAX stability and antagonizing apoptosis
| ||
Cell Death Dis ILF3 promotes colorectal cancer cell resistance to ferroptosis by enhancing cysteine uptake and GSH synthesis via stabilizing SLC3A2 mRNA. | ||
J Cell Mol Med Jianpi Jiedu Xiaozheng Fang Regulates Hepatocellular Carcinoma Proliferation and Metastasis Based on Network Pharmacology. | ||
J Biol Chem Fumarate hydratase suppresses glioblastoma progression by restricting fumarate-mediated succination of TRIM17. |
